A genetic method for diagnosing lantana poisoning using a barcoding-based, genusspecific, polymerase chain reaction
INTRODUCTION: Lantana species are highly toxic as they contain lantadenes and other chemical compounds. While human poisonings have occasionally been reported, cattle throughout the world are often poisoned, which represents a serious loss of income in many countries. In order to reduce the mortality rate among lantana-poisoned animals, an early and reliable diagnosis is required. Our aim, therefore, is to develop a genetic test to determine the presence of Lantana Camara and other common lantana species in the content of the digestive tube and faecal products of cattle. MATERIAL AND METHODS: A comparison between Internal Transcribed Spacer 1, 2 and 5.8 subunit DNA sequences of Lantana Camara and all other species available in NCBI Nucleotide database has been undertaken using the BLAST tool. As a result, several DNA fragments, highly specific for Lantana were identified. PCR primers were designed to hybridize on these sequences. Finally, a pair of universal primers was used in order to achieve a constant band for all angiosperms. RESULTS: Our multiplex PCR design generated a constant band that certified the correct development of the PCR in all angiosperms tested. However, in the case of Lantana Camara DNA, a specific and lighter band was generated, allowing the positive identification of this species. DISCUSSION: Ours is the first specific test for confirming Lantana Camara poisoning reported to date, and may provide a useful tool to veterinary services in countries where lantana poisoning in cattle is frequent.
Francès, Francesc. A genetic method for diagnosing lantana poisoning using a barcoding-based, genusspecific, polymerase chain reaction. En: Gaceta internacional de ciencias forenses, 2014, No. 13: 11