Activation of bee venom phospholipase A2 through a peptide-enzyme complex

Abstract
Phospholipase A2 activation by membrane‐bound peptides was investigated in order to understand the role of the membrane‐induced conformation on activation, and to examine the occurrence of a peptide‐enzyme complex at the lipid/water interface. For the peptides studies, bee venom phospholipase A2 was stimulated regardless of the membrane‐bound conformation (α‐helix, β‐sheet or random coil). Using antisera raised against melittin, we were able to demonstrate the occurrence of a calcium‐dependent complex involving the enzyme, phospholipid substrate, and peptide.
Description
Bibliographic reference
Mingarro Muñoz, Ismael Pérez Payá, Enrique Pinilla, Clemencia Appel, Jon R. Houghten, Richard A. Blondelle, Sylvie E. 1995 Activation of bee venom phospholipase A2 through a peptide-enzyme complex Febs Letters 372 1 131 134