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Quantification of rifampicin and rifabutin in plasma of tuberculosis patients by micellar liquid chromatography

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Quantification of rifampicin and rifabutin in plasma of tuberculosis patients by micellar liquid chromatography

dc.contributor.author	Goberna Bravo, María Ángeles
dc.contributor.author	Peris Vicente, Juan
dc.contributor.author	Esteve i Romero, Josep-Salvador
dc.contributor.author	Mishra, Pooja
dc.contributor.author	Albiol-Chiva, Jaume
dc.date.accessioned	2023-11-20T14:52:34Z
dc.date.available	2023-11-20T14:52:34Z
dc.date.issued	2020
dc.identifier.uri	https://hdl.handle.net/10550/91262
dc.description.abstract	A Micellar Liquid Chromatographic method is described to determine Rifampicin and Rifabutin in plasma from Tuberculosis patients. Samples were diluted in mobile phase and then directly injected, avoiding long and tedious extraction steps. The analytes were resolved from the matrix without interferences from endogenous compounds using a mobile phase of sodium dodecyl sulfate 0.15 mol L-1-6%(v/v) 1-pentanol and phosphate buffer at pH 3, running at 1 mL min−1 through a C18 column at 25 °C. Detection was carried out by UV absorbance at 270 nm. Under these conditions, the final chromatographic analysis time was 22 min. The analytical methodology was validated following the FDA 2018 Bioanalytical Method Validation Guidance for Industry. The response of the drugs in plasma was linear in the 0.05-5 μg/mL range, with r2 > 0.9993. Trueness and precision were <14% for both substances. Carry over and matrix effects were negligible. Dilution integrity, robustness and stability were also investigated. Method was reliable, economic, eco-friendly, safe, easy-to-conduct, and with a high sample throughput, thus useful for routine analysis. Finally, the analytical method was used to determine both antituberculosis drugs in incurred plasma samples of Tuberculosis patients.
dc.language.iso	eng
dc.relation.ispartof	Microchemical Journal, 2020, vol. 157, p. 104865
dc.source	Goberna Bravo, María Ángeles, Peris Vicente, Juan, Esteve i Romero, Josep-Salvador, Mishra, Pooja, Albiol-Chiva, Jaume (2020). Quantification of rifampicin and rifabutin in plasma of tuberculosis patients by micellar liquid chromatography. Microchemical Journal, 157 104865. https://doi.org/10.1016/j.microc.2020.104865
dc.subject	tuberculosi
dc.subject	plasma (gasos ionitzats)
dc.subject	química analítica
dc.title	Quantification of rifampicin and rifabutin in plasma of tuberculosis patients by micellar liquid chromatography
dc.type	journal article
dc.date.updated	2023-11-20T14:52:34Z
dc.identifier.doi	10.1016/j.microc.2020.104865
dc.identifier.idgrec	139729
dc.rights.accessRights	open access