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Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction : Comparison at Acute Event and One-Year Follow-Up

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Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction : Comparison at Acute Event and One-Year Follow-Up

dc.contributor.author	Mompeón, Ana
dc.contributor.author	Pérez Cremades, Daniel
dc.contributor.author	Paes, Ana B.
dc.contributor.author	Sanchis Forés, Juan
dc.contributor.author	Ortega-Paz, Luis
dc.contributor.author	Rut, Andrea
dc.contributor.author	Brugaletta, Salvatore
dc.contributor.author	Sabate, Manel
dc.contributor.author	Novella, Susana
dc.contributor.author	Dantas, A. P.
dc.contributor.author	Hermenegildo, Carlos
dc.date.accessioned	2023-05-15T18:30:53Z
dc.date.available	2023-05-15T18:30:53Z
dc.date.issued	2022
dc.identifier.citation	Mompeón, Ana Pérez Cremades, Daniel Paes, Ana B. Sanchis Forés, Juan Ortega-Paz, Luis Rut, Andrea Brugaletta, Salvatore Sabate, Manel Novella, Susana Dantas, A. P. Hermenegildo, Carlos 2022 Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction : Comparison at Acute Event and One-Year Follow-Up Cells 11 11 1823
dc.identifier.uri	https://hdl.handle.net/10550/86622
dc.description.abstract	MicroRNAs (miRNA) are major regulators of intercellular communication and key players in the pathophysiology of cardiovascular disease. This study aimed to determine the miRNA fingerprint in a cohort of 53 patients with acute myocardial infarction (AMI) with non-ST-segment elevation (NSTEMI) relative to miRNA expression in healthy controls (n = 51). miRNA expression was initially profiled by miRNA array in the serum of patients undergoing cardiac catheterization during NSTEMI (n = 8) and 1 year past the event (follow-up, n = 8) and validated in the entire cohort. In total, 58 miRNAs were differentially expressed during AMI (p < 0.05), while 36 were modified at follow-up (Fisher's exact test: p = 0.0138). Enrichment analyses revealed differential regulation of biological processes by miRNA at each specific time point (AMI vs. follow-up). During AMI, the miRNA profile was associated mainly with processes involved in vascular development. However, 1 year after AMI, changes in miRNA expression were partially related to the regulation of cardiac tissue morphogenesis. Linear correlation analysis of miRNA with serum levels of cytokines and chemokines revealed that let-7g-5p, let-7e-5p, and miR-26a-5p expression was inversely associated with serum levels of pro-inflammatory cytokines TNF- , and the chemokines MCP-3 and MDC. Transient transfection of human endothelial cells (HUVEC) with let-7e-5p inhibitor or mimic demonstrated a key role for this miRNA in endothelial function regulation in terms of cell adhesion and angiogenesis capacity. HUVEC transfected with let-7e-5p mimic showed a 20% increase in adhesion capacity, whereas transfection with let-7e-5p inhibitor increased the number of tube-like structures. This study pinpoints circulating miRNA expression fingerprint in NSTEMI patients, specific to the acute event and changes at 1-year follow-up. Additionally, given its involvement in modulating endothelial cell function and vascularization, altered let-7e-5p expression may constitute a therapeutic biomarker and target for ischemic heart disease.
dc.language.iso	eng
dc.relation.ispartof	Cells, 2022, vol. 11, num. 11, p. 1823
dc.subject	Fisiologia cel·lular
dc.subject	Malalties coronàries
dc.title	Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction : Comparison at Acute Event and One-Year Follow-Up
dc.type	journal article
dc.date.updated	2023-05-15T18:30:53Z
dc.identifier.doi	10.3390/cells11111823
dc.identifier.idgrec	154443
dc.rights.accessRights	open access