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Plasticity of cell proliferation in the retina of Austrolebias charrua fish under light and darkness conditions

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Plasticity of cell proliferation in the retina of Austrolebias charrua fish under light and darkness conditions

dc.contributor.author	Berrosteguieta, Inés
dc.contributor.author	Rosillo, Juan Carlos
dc.contributor.author	Herrera, María Laura
dc.contributor.author	Olivera-Bravo, Silvia
dc.contributor.author	Casanova, Gabriela
dc.contributor.author	Herranz Pérez, Vicente
dc.contributor.author	García Verdugo, José Manuel
dc.contributor.author	Fernández, Anabel Sonia
dc.date.accessioned	2023-01-20T14:00:34Z
dc.date.available	2023-01-20T14:00:34Z
dc.date.issued	2022
dc.identifier.uri	https://hdl.handle.net/10550/85062
dc.description.abstract	Austrolebias annual fishes exhibit cell proliferation and neurogenesis throughout life. They withstand extreme environmental changes as their habitat dries out, pressuring nervous system to adapt. Their visual system is challenged to adjust as the water becomes turbid. Therefore, this study focused on how change in photic envi- ronment can lead to an increased cell proliferation in the retina. We administered 5-chloro-2′- deoxyuridine (CldU) and 5-iodo-2′-deoxyuridine (IdU) at different temporal windows to detect cell proliferation in natural light and permanent darkness. Stem/progenitor cells were recognized as IdU+/CldU+ nuclei co-labeled with Sox2, Pax6 or BLBP found in the ciliary marginal zone (CMZ). The expression pattern of BLBP + glial cells and ultrastructural analysis indicates that CMZ has different cell progenitors. In darkness, the number of dividing cells significantly increased, compared to light conditions. Surprisingly, CMZ IdU+/CldU + cell number was similar under light and darkness, suggesting a stable pool of stem/progenitor cells possibly responsible for retinal growth. Therefore, darkness stimulated cell progenitors outside the CMZ, where Müller glia play a crucial role to generate rod precursors and other cell types that might integrate rod-dependent circuits to allow darkness adaptation. Thus, the Austrolebias fish retina shows great plasticity, with cell proliferation rates significantly higher than that of brain visual areas.
dc.language.iso	eng
dc.relation.ispartof	Current Research in Neurology, 2022, vol. 3, num. 100042, p. 1-16
dc.source	Berrosteguieta, Inés Rosillo, Juan Carlos Herrera, María Laura Olivera-Bravo, Silvia Casanova, Gabriela Herranz Pérez, Vicente García Verdugo, José Manuel Fernández, Anabel Sonia 2022 Plasticity of cell proliferation in the retina of Austrolebias charrua fish under light and darkness conditions Current Research in Neurology 3 100042 1 16
dc.subject	Cèl·lules mare
dc.subject	Peixos
dc.subject	Neurociències
dc.title	Plasticity of cell proliferation in the retina of Austrolebias charrua fish under light and darkness conditions
dc.type	journal article	es_ES
dc.date.updated	2023-01-20T14:00:36Z
dc.identifier.doi	https://doi.org/10.1016/j.crneur.2022.100042
dc.identifier.idgrec	156475
dc.rights.accessRights	open access	es_ES