NAGIOS: RODERIC FUNCIONANDO

Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes

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Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes

dc.contributor.author	Picazo Campos, Cecilia
dc.contributor.author	Matallana Redondo, Emilia
dc.contributor.author	Aranda Fernández, Agustín
dc.date.accessioned	2019-05-09T14:36:26Z
dc.date.available	2019-05-09T14:36:26Z
dc.date.issued	2018
dc.identifier.uri	http://hdl.handle.net/10550/70123
dc.description.abstract	The thioredoxin system plays a predominant role in the control of cellular redox status. Thioredoxin reductase fuels the system with reducing power in the form of NADPH. The TORC1 complex promotes growth and protein synthesis when nutrients, particularly amino acids, are abundant. It also represses catabolic processes, like autophagy, which are activated during starvation. We analyzed the impact of yeast cytosolic thioredoxin reductase TRR1 deletion under different environmental conditions. It shortens chronological life span and reduces growth in grape juice fermentation. TRR1 deletion has a global impact on metabolism during fermentation. As expected, it reduces oxidative stress tolerance, but a compensatory response is triggered, with catalase and glutathione increasing. Unexpectedly, TRR1 deletion causes sensitivity to the inhibitors of the TORC1 pathway, such as rapamycin. This correlates with low Tor2p kinase levels and indicates a direct role of Trr1p in its stability. Markers of TORC1 activity, however, suggest increased TORC1 activity. The autophagy caused by nitrogen starvation is reduced in the trr1Δ mutant. Ribosomal protein Rsp6p is dephosphorylated in the presence of rapamycin. This dephosphorylation diminishes in the TRR1 deletion strain. These results show a complex network of interactions between thioredoxin reductase Trr1p and the processes controlled by TOR.
dc.language.iso	eng
dc.relation.ispartof	Scientific Reports, 2018, vol. 8, p. 16500
dc.rights.uri	info:eu-repo/semantics/openAccess
dc.source	Picazo Campos, Cecilia Matallana Redondo, Emilia Aranda Fernández, Agustín 2018 Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes Scientific Reports 8 16500
dc.subject	Estrès oxidatiu
dc.subject	Microbiologia
dc.title	Yeast thioredoxin reductase Trr1p controls TORC1-regulated processes
dc.type	info:eu-repo/semantics/article
dc.date.updated	2019-05-09T14:36:27Z
dc.identifier.doi	https://doi.org/10.1038/s41598-018-34908-4
dc.identifier.idgrec	129443