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Stabilization process in Saccharomyces intra and interspecific hybrids in fermentative conditions

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Stabilization process in Saccharomyces intra and interspecific hybrids in fermentative conditions

dc.contributor.author	Pérez Través, Laura
dc.contributor.author	Lopes, Christian A.
dc.contributor.author	Barrio Esparducer, Eladio
dc.contributor.author	Querol Simón, Amparo
dc.date.accessioned	2016-06-14T14:20:55Z
dc.date.available	2016-06-14T14:20:55Z
dc.date.issued	2014
dc.identifier.citation	Pérez Través, Laura Lopes, Christian A. Barrio Esparducer, Eladio Querol Simón, Amparo 2014 Stabilization process in Saccharomyces intra and interspecific hybrids in fermentative conditions International Microbiology 17 4 213 224
dc.identifier.uri	http://hdl.handle.net/10550/54060
dc.description.abstract	We evaluated the genetic stabilization of artificial intra- (Saccharomyces cerevisiae) and interspecific (S. cerevisiae × S. kudriavzevii) hybrids under wine fermentative conditions. Large-scale transitions in genome size and genome reorganizations were observed during this process. Interspecific hybrids seem to need fewer generations to reach genetic stability than intraspecific hybrids. The largest number of molecular patterns recovered among the derived clones was observed for intraspecific hybrids, particularly for those obtained by rare-mating. Molecular marker analyses revealed that unstable clones could change during the industrial process to obtain active dry yeast. When no changes in molecular markers and ploidy were observed after this process, no changes in genetic composition were confirmed by comparative genome hybridization, considering the clone as a stable hybrid. According to our results, under these conditions, fermentation steps 3 and 5 (30-50 generations) would suffice to obtain genetically stable interspecific and intraspecific hybrids, respectively.
dc.language.iso	eng
dc.relation.ispartof	International Microbiology, 2014, vol. 17, num. 4, p. 213-224
dc.subject	Fermentació
dc.subject	Aliments Microbiologia
dc.subject	Genomes
dc.title	Stabilization process in Saccharomyces intra and interspecific hybrids in fermentative conditions
dc.type	journal article	es_ES
dc.date.updated	2016-06-14T14:20:56Z
dc.identifier.doi	10.2436/20.1501.01.224
dc.identifier.idgrec	111909
dc.rights.accessRights	open access	es_ES