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Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups☆

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Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups☆

dc.contributor.author	Escobar, Javier	es_ES
dc.contributor.author	Cubells, Elena	es_ES
dc.contributor.author	Enomoto, Masahiro	es_ES
dc.contributor.author	Quintás Alonso, Guillermo	es_ES
dc.contributor.author	Kuligowski, Julia	es_ES
dc.contributor.author	Martínez Fernández, Cristina	es_ES
dc.contributor.author	Torres-Cuevas, Isabel	es_ES
dc.contributor.author	Sastre, Juan	es_ES
dc.contributor.author	Belik, Jaques	es_ES
dc.contributor.author	Vento Torres, Máximo	es_ES
dc.date.accessioned	2015-06-19T07:47:46Z
dc.date.available	2015-06-19T07:47:46Z
dc.date.issued	2013	es_ES
dc.identifier.citation	Redox Biology Vol. 1 Issue 1: pp. 297-303	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44511
dc.description.abstract	BackgroundFetal-to-neonatal transition is associated with oxidative stress. In preterm infants, immaturity of the antioxidant system favours supplemental oxygen-derived morbidity and mortality.ObjectivesTo assess if prolonging in utero-like oxygenation during the fetal-to-neonatal transition limits oxidative stress in the lung and brain, improving postnatal adaptation of mice pups.Material and methodsInspiratory oxygen fraction (FiO2) in pregnant mice was reduced from 21% (room air) to 14% (hypoxia) 8–12 h prior to delivery and reset to 21% 6–8 h after birth. The control group was kept at 21% during the procedure. Reduced (GSH) and oxidized (GSSG) glutathione and its precursors [γ-glutamyl cysteine (γ-GC) and L-cysteine (CySH)] content and expression of several redox-sensitive genes were evaluated in newborn lung and brain tissue 1 (P1) and 7 (P7) days after birth.ResultsAs compared with control animals, the GSH/GSSG ratio was increased in the hypoxic group at P1 and P7 in the lung, and at P7 in the brain. In the hypoxic group a significant increase in the mRNA levels of NAD(P)H:quinone oxidoreductase 1 (noq1), Sulfiredoxin 1 (srnx1) and Glutathione Peroxidase 1 (gpx) was found in lung tissue at P1, as well as a significant increase in gpx in brain tissue at P7.ConclusionsDelaying the increase in tissue oxygenation to occur after birth reduces short-and-long-term oxidative stress in the lung. Similar yet more subtle effects were found in the brain. Apparently, the fetal-to-neonatal transition under hypoxic conditions appears to have protective qualities.	es_ES
dc.subject	CySH (l-cysteine)	es_ES
dc.subject	CyS–NEM (cysteine covalently bonded to N-ethylmaleimide)	es_ES
dc.subject	FiO2 (inspiratory oxygen fraction)	es_ES
dc.subject	gapdh (glyceraldehyde-3-phosphate dehydrogenase gene)	es_ES
dc.subject	GCL (glutamylcysteine ligase)	es_ES
dc.subject	gclm (glutamylcysteine ligase modifier subunit gene)	es_ES
dc.subject	γ-GC (gamma-glutamyl cysteine)	es_ES
dc.subject	γ-GC–NEM (gamma-glutamyl cysteine covalently bonded to N-ethylmaleimide)	es_ES
dc.subject	gpx1 (glutathione peroxidase 1 gene)	es_ES
dc.subject	GSH (reduced glutathione)	es_ES
dc.subject	GS–NEM (reduced glutathione covalently bonded to N-ethylmaleimide)	es_ES
dc.subject	gsr (glutathione reductase gene)	es_ES
dc.subject	GSSG (oxidized glutathione)	es_ES
dc.subject	G18 (18th day of gestation)	es_ES
dc.subject	g6pdx (glucose 6 phosphate dehydrogenase gene)	es_ES
dc.subject	LC–MS/MS (liquid chromatography coupled to tandem mass spectrometry)	es_ES
dc.subject	m/z (mass-to-charge ratio)	es_ES
dc.subject	noq1 (NAD(P)H:quinone oxidoreductase 1)	es_ES
dc.subject	me1 (malic enzyme 1 gene)	es_ES
dc.subject	NEM (N-ethylmaleimide)	es_ES
dc.subject	O14 (hypoxia group FiO2=14%)	es_ES
dc.subject	O21 (normoxia group FiO2=21%)	es_ES
dc.subject	paO2 (partial pressure of oxygen)	es_ES
dc.subject	pgd (phosphogluconate dehydrogenase gene)	es_ES
dc.subject	P1 (24 h after birth)	es_ES
dc.subject	P7 (1 week after birth)	es_ES
dc.subject	SpO2 (oxygen saturation)	es_ES
dc.subject	srnx1 (sulfiredoxin 1 gene)	es_ES
dc.subject	trxnd1 (thioredoxin reductase 1 gene)	es_ES
dc.subject	fetal-to-neonatal transition	es_ES
dc.subject	oxygen	es_ES
dc.subject	oxidative stress	es_ES
dc.subject	redox regulation	es_ES
dc.subject	glutathione	es_ES
dc.title	Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups☆	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.1016/j.redox.2013.04.002	es_ES
dc.identifier.idgrec	097581	es_ES