Genomic investigation of a legionellosis outbreak in a persistently colonized hotel

Roderic Mòbil

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Genomic investigation of a legionellosis outbreak in a persistently colonized hotel

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dc.contributor.author Sánchez-Busó, Leonor
dc.contributor.author Guiral, Silvia
dc.contributor.author Crespi, Sebastián
dc.contributor.author Moya, Víctor
dc.contributor.author Camaró, María L.
dc.contributor.author Olmos, María P.
dc.contributor.author Adrián, Francisco
dc.contributor.author Morera, Vicente
dc.contributor.author González-Morán, Francisco
dc.contributor.author Vanaclocha, Hermelinda
dc.contributor.author González Candelas, Fernando
dc.date.accessioned 2017-05-19T15:20:05Z
dc.date.available 2017-05-19T15:20:05Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/10550/58578
dc.description.abstract Objectives: A long-lasting legionellosis outbreak was reported between November 2011 and July 2012 in a hotel in Calpe (Spain) affecting 44 patients including six deaths. Intensive epidemiological and microbiological investigations were performed in order to detect the reservoirs. Methods: Clinical and environmental samples were tested for the presence and genetic characterization of Legionella pneumophila. Six of the isolates were subjected to whole-genome sequencing. Results: Sequencing of 14 clinical and 260 environmental samples revealed sequence type (ST) 23 as the main responsible strain for the infections. This ST was found in the spa pool, from where it spread to other hotel public spaces, explaining the ST23 clinical cases, including guests who had not visited the spa. Uncultured clinical specimens showed profiles compatible with ST23, ST578, and mixed patterns. Profiles compatible with ST578 were obtained by direct sequencing from biofilm samples collected from the domestic water system, which provided evidence for the source of infection for non ST23 patients. Whole genome data from five ST23 strains and the identification of different STs and Legionella species showed that different hotel premises were likely colonized since the hotel opening thus explaining how different patients had been infected by distinct STs. Conclusions: Both epidemiological and molecular data are essential in the investigation of legionellosis outbreaks. Whole-genome sequencing data revealed significant intra-ST variability and allowed to make further inference on the short-term evolution of a local colonization of L. pneumophila.
dc.language.iso eng
dc.relation.ispartof Frontiers In Microbiology, 2016, vol. 6, p. 1556
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Sánchez-Busó, Leonor Guiral, Silvia Crespi, Sebastián Moya, Víctor Camaró, María L. Olmos, María P. Adrián, Francisco Morera, Vicente González-Morán, Francisco Vanaclocha, Hermelinda González Candelas, Fernando 2016 Genomic investigation of a legionellosis outbreak in a persistently colonized hotel Frontiers In Microbiology 6 1556
dc.subject Microbiologia
dc.subject Legionel·losi
dc.subject Salut pública
dc.title Genomic investigation of a legionellosis outbreak in a persistently colonized hotel
dc.type info:eu-repo/semantics/article
dc.date.updated 2017-05-19T15:20:05Z
dc.identifier.doi https://doi.org/10.3389/fmicb.2015.01556
dc.identifier.idgrec 117889


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Roderic Mòbil